Publication

Using stereochemical insights and synthetic chemistry principles, we successfully achieved the total synthesis of two diastereomers of thermolide D, as well as the proposed structure of thermolide E. By comparing their NMR spectra and demonstrating coelution of the synthetic and natural products on a semipreparative HPLC column, we confirmed the correct structures of thermolides D and E. Additionally, the stereochemical approach used in this study provides a reliable framework for future structural determinations of fungal-derived polyketides.